APPLICATION OF CONFOCAL IMAGING AND IMMUNOHISTOCHEMISTRY TO STUDY LOCAL ALDEHYDE-PRODUCING LIPIDS ON ?-AMYLOID CLEARANCE IN MICE

Semyachkina-Glushkovskaya Oxana V.  -  Doctor of Biological Sciences, Associate Professor, Head of the Department of Human and Animal Physiology, Saratov National Research State University named after N.G. Chernyshevsky, Russian Federation, 410012, Saratov, Astrakhanskaya str., 83, glushkovskaya@mail.ru

Sarantseva Elena Ivanovna  -  candidate of biological sciences, Associate Professor, Department of Human and Animal Physiology, Saratov National Research State University named after N.G. Chernyshevsky, Russin Federation, 410012, Saratov, st. Astrakhanskaya, 83, sophora@mail.ru

Iskra Tatyana Dmitrievna  -  candidate of biological sciences, Associate Professor, Department of Human and Animal Physiology, Saratov National Research State University named after N.G. Chernyshevsky, Russian Federation, 410012, Saratov, st. Astrakhanskaya, 83, tata-isk@yandex.ru

Study conditions that ensure brain tissuesclearance from metabolites and toxins are getting more relevant within the context of the study of neurological diseases and pathophysiological conditions. Disruption of clearance processes can lead to inflammation, neurodegeneration and other complications. The research problems are related to neuroimaging, which allowsstudying various structures of the brain limited by the skull bones. In this regard, one of the main tasks of modern neuroscience is the search for visualizationmethods of brain structures allowingtoimproveits tissues’ clearance. In this work we studied the effect of plasmalogens on the lymphatic clearance of ?-amyloid from the brain of mice with Alzheimer's disease and mice of different age using confocal microscopy and immunohistochemistry. ?-Amyloid is taken up by neurons as a result of metabolism and plays a major role in maintaining homeostasis. ?-amyloid in the normformed in the brain is excreted by lymph through the meningeal vessels into the deep cervical nodes. However, with age and during the development of Alzheimer's disease, we observe excessive accumulation of ?-amyloid in the brain, which leads to neurodegenerative disorders. According to new data, plasmalogens, which are one of the key lipids of the brain, may be useful in AD and cognitive aging. The results show that Pls effectively increases the level of A? in the brain. Thus, the use of confocal imaging may be a promising for alternative or adjunctive therapy for Alzheimer's and age-related brain diseases used to improve lymphatic clearance of A?.

Key words: confocal microscopy, aldehyde-producing lipids, Alzheimer's disease, ?-amyloid, lymphatic clearance