THE EFFECT OF AGONISTS AND BLOCKERS OF ADRENERGIC, SEROTONINERGIC AND DOPAMINERGIC RECEPTORS ON OSMOTIC AND PEROXIDATION HEMOLYSIS OF ERYTHROCYTES IN RATS

Kuryanova Evgenia Vladimirovna  -  Doctor of Biological Sciences, Associate Professor, Professor of the Department of Fundamental Biology, Astrakhan State University named by V.N. Tatischev, Russia, 414000, Astrakhan, Shaumyan Sq.,1, teplyconf@yandex.ru

We studied the effect of substances, complementary to adreno-, serotonin- and dopamine receptors in the incubating medium, on the intensity of osmotic and peroxide hemolysis of erythrocytes. The intensity of osmotic hemolysis of erythrocytes was determined in the presence of adrenaline (4*10-6 g/ml), serotonin (12*10-6 g/ml), dopamine (4*10-6 g/ml, anaprilin - a blocker of adrenoreceptors - (2.83 * 10-4 g / ml), promethazine - a serotonin receptor blocker - (12 * 10-6 g / ml), sulpiride - a dopamine receptor blocker - (6 * 10-5 g /ml) in the incubation medium and compared with its The intensity of peroxide hemolysis of erythrocytes was assessed in the presence of adrenaline (12*10-6 g/ml), serotonin (38*10-6 g/ml), dopamine - 8*10-6 g/ml), anaprilin (8.82*10-4 g/ml), promethazine (38*10-6 g/ml), sulpiride (18*10-5 g/ml) in comparison with control samples. The concentrations of active substances were calculated taking into account the volume of the sample and the concentration of erythrocytes in them. Substances complementary to adreno-, serotonino-, dopamine receptors in the incubation medium reduce the intensity of osmotic hemolysis and increase peroxide hemolysis. Blockers that inhibit osmotic hemolysis by 35-45% have a greater effect. A sharp increase in peroxide hemolysis occurs in the presence of anaprilin, moderate - in the presence of sulpiride, inhibition of hemolysis - in the presence of promethazine. The results indicate the presence of receptors for various monoamines on erythrocyte membranes and are important for the development of methods for monitoring the effects of drugs that affect neurotransmitter processes.

Key words: adrenaline, serotonin, dopamine, anaprilin, prometazine, sulpiride, osmotic erythrocyte hemolysis, peroxide hemolysis of erythrocytes, rats