THE LEVEL OF FREE RADICAL OXIDATION OF THE BLOOD OF PRENATALLY STRESSED FEMALE RATS AT DIFFERENT STAGES OF THE ESTROUS CYCLE

Kuleshova Olga N.  -  Ph. D. (Biology), Senior Researcher, Astrakhan State University, 1 Shaumyana Sq., Astrakhan, 414000, Russian Federation, pozdniakova_olga@list.ru

Brykova Anastasia N.  -  student, Astrakhan State University, 1 Shaumyana Sq., Astrakhan, 414000, Russian Federation,

Yakovenkova Lyudmila A.  -  Ph. D. (Biology), Associate Professor, Astrakhan State University, 1 Shaumyana Sq., Astrakhan, 414000, Russian Federation, lykovenkova79@mail.ru

The level of oxidative modification of proteins (OMP) and lipid peroxidation (POL) in blood plasma and erythrocyte hemolysate of sexually mature prenatally stressed female rats at the estrus and diestrus stages of ovulation cycle was studied. Prenatal stress (3-hour immobilization) was carried out in the period from 16 to 19 days of prenatal ontogenesis. The state of free radical homeostasis (SRO) was assessed for 3-4 months of postnatal ontogenesis. After undergoing prenatal stress, more significant shifts in SR homeostasis were observed in the plasma of female rats than in erythrocytes. In general, an increase in the level of POL and a decrease in OMP products are characteristic. However, the level of full lipids in erythrocytes remained unchanged. Such changes may be associated with different levels of activity of different parts of the antioxidant system. It was also noted that POL at the diestrus stage remains normal (the level of the control group), while significant shifts in this parameter were noted for the estrus stage, which most likely depended on changes in hormonal activity that shifted after the prenatal stress.

Key words: free radical oxidation, oxidative modification of proteins, lipid peroxidation, structural and reserve lipids, prenatal stress, female rats, plasma